Quantitative Western Blot

Western blotting can be used to monitor protein levels, post-translational modifications, and to understand the mechanism of action for drug candidates. Protein abundance is evaluated by in lane normalization to a housekeeping protein, when possible.

Figure 1: Detection and analysis of Tau protein in rTg4510, a mouse model of tauopathy, by quantitative western blotting. Tau protein was most abundant in rTg4510 mouse brains at 6 months of age as compared to that of age-matched tTA control mice. Tau detection was carried out with ECL Plus chemifluorescence (FAM) and GAPDH, a housekeeping protein, was detected by direct fluores cence with rhodamine-conjugated GAPDH antibody.